Essential Oils from Humulus Lupulus scCO₂ Extract by Hydrodistillation and Microwave-Assisted Hydrodistillation.
Katarzyna TyśkiewiczRoman GieysztorMarcin KonkolJan SzałasEdward RójPublished in: Molecules (Basel, Switzerland) (2018)
Two different extraction methods were used for a comparative study of essential oils obtained from the Humulus lupulus scCO₂ (sc-supercritical) extract: microwave-assisted hydrodistillation (MAHD) and conventional hydrodistillation (HD). As a result, the best conditions for the maximum essential oil production were determined for the MAHD method at 335 W microwave power for 30 min at water to raw material ratio of 8:3. The obtained essential oils were enriched in β-myrcene in the amount of 74.13%⁻89.32% (wt%). Moreover, the first application for determination of the above-mentioned volatile compounds by supercritical fluid chromatography (SFC) with photo-diode array detection (PDA) is presented, which in comparison with gas chromatography coupled with mass spectrometry (GC-MS/MS) resulted in similar values for β-myrcene and α-humulene in obtained samples within less than 1 min.
Keyphrases
- gas chromatography
- mass spectrometry
- tandem mass spectrometry
- ultra high performance liquid chromatography
- liquid chromatography
- solid phase extraction
- high performance liquid chromatography
- essential oil
- high resolution mass spectrometry
- ms ms
- high resolution
- gas chromatography mass spectrometry
- oxidative stress
- simultaneous determination
- liquid chromatography tandem mass spectrometry
- capillary electrophoresis
- molecularly imprinted
- anti inflammatory
- real time pcr
- label free
- loop mediated isothermal amplification
- clinical evaluation