Immobilized Keratin HPLC Stationary Phase-A Forgotten Model of Transdermal Absorption: To What Molecular and Biological Properties Is It Relevant?
Anna W Weronika SobanskaBrzezińska ElżbietaPublished in: Pharmaceutics (2023)
Chromatographic retention data collected on immobilized keratin (KER) or immobilized artificial membrane (IAM) stationary phases were used to predict skin permeability coefficient (log K p ) and bioconcentration factor (log BCF ) of structurally unrelated compounds. Models of both properties contained, apart from chromatographic descriptors, calculated physico-chemical parameters. The log K p model, containing keratin-based retention factor, has slightly better statistical parameters and is in a better agreement with experimental log K p data than the model derived from IAM chromatography; both models are applicable primarily to non-ionized compounds.Based on the multiple linear regression (MLR) analyses conducted in this study, it was concluded that immobilized keratin chromatographic support is a moderately useful tool for skin permeability assessment.However, chromatography on immobilized keratin may also be of use for a different purpose-in studies of compounds' bioconcentration in aquatic organisms.
Keyphrases
- simultaneous determination
- liquid chromatography
- ionic liquid
- mass spectrometry
- tandem mass spectrometry
- high performance liquid chromatography
- capillary electrophoresis
- magnetic nanoparticles
- ms ms
- high speed
- soft tissue
- electronic health record
- machine learning
- wound healing
- endothelial cells
- solid phase extraction
- magnetic resonance
- data analysis
- artificial intelligence
- single molecule
- gram negative