Orientation-Independent-DIC imaging reveals that a transient rise in macromolecular crowding contributes to mitotic chromosome condensation.

Shiori Iida Satoru Ide Sachiko Tamura Tomomi Tani Tatsuhiko Goto Michael Shribak Kazuhiro Maeshima

Published in: bioRxiv : the preprint server for biology (2023)

, higher concentrations of macromolecules condense chromatin and make it stiffer and more solid-like. Our results suggest that the rise in macromolecular crowding renders chromosomes more rigid, ensuring accurate chromosome transmission during cell division.

Keyphrases

high resolution
copy number
single cell
gene expression
dna damage
genome wide
transcription factor
cell therapy
cell cycle
cerebral ischemia
cell proliferation
fluorescence imaging