Neuropathological Applications of Microscopy with Ultraviolet Surface Excitation (MUSE): A Concordance Study of Human Primary and Metastatic Brain Tumors.
Mirna LechpammerAustin ToddVivian TangTaryn MorningstarAlexander BorowskyKiarash ShahlaieJohn A KintnerJohn D McPhersonJohn W BishopFarzad FereidouniZachary T HarmanyNicholas ColeyDavid ZagzagJason W H WongJiang TaoLuke B HessonLeslie BurnettRichard LevensonPublished in: Brain sciences (2024)
Whereas traditional histology and light microscopy require multiple steps of formalin fixation, paraffin embedding, and sectioning to generate images for pathologic diagnosis, Microscopy using Ultraviolet Surface Excitation (MUSE) operates through UV excitation on the cut surface of tissue, generating images of high resolution without the need to fix or section tissue and allowing for potential use for downstream molecular tests. Here, we present the first study of the use and suitability of MUSE microscopy for neuropathological samples. MUSE images were generated from surgical biopsy samples of primary and metastatic brain tumor biopsy samples (n = 27), and blinded assessments of diagnoses, tumor grades, and cellular features were compared to corresponding hematoxylin and eosin (H&E) images. A set of MUSE-treated samples subsequently underwent exome and targeted sequencing, and quality metrics were compared to those from fresh frozen specimens. Diagnostic accuracy was relatively high, and DNA and RNA integrity appeared to be preserved for this cohort. This suggests that MUSE may be a reliable method of generating high-quality diagnostic-grade histologic images for neuropathology on a rapid and sample-sparing basis and for subsequent molecular analysis of DNA and RNA.
Keyphrases
- single molecule
- optical coherence tomography
- high resolution
- deep learning
- convolutional neural network
- high speed
- small cell lung cancer
- high throughput
- endothelial cells
- fine needle aspiration
- label free
- clinical trial
- cell free
- ultrasound guided
- nucleic acid
- minimally invasive
- energy transfer
- circulating tumor
- single cell
- gene expression
- machine learning
- tandem mass spectrometry
- induced pluripotent stem cells
- climate change
- robot assisted
- human health
- liquid chromatography