True or false: what are the factors that influence COVID-19 diagnosis by RT-qPCR?
Luina Benevides LimaFelipe Pantoja MesquitaLais Lacerda Brasil de OliveiraFrancisca Andréa da Silva OliveiraMaria Elisabete Amaral de MoraesPedro Filho Noronha SouzaRaquel Carvalho MontenegroPublished in: Expert review of molecular diagnostics (2022)
Here is highlighted and discussed several factors that can interfere in the RT-PCR analysis, such as the viral load of the sample, collection site, collection methodology, sample storage, transport, primer, and probe mismatch/dimerization in different brand kits. This is a pioneer study to discuss the factor that could lead to the wrong interpretation of RT-qPCR diagnosis of SARS-CoV-2. This study aimed to help the readers to understand what very likely is behind a bad result of SARS-CoV-2 detection by RT-PCR and what could be done to reach a reliable diagnosis.