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Aptamer-Functionalized Ce 4+ -Ion-Modified C-Dots: Peroxidase Mimicking Aptananozymes for the Oxidation of Dopamine and Cytotoxic Effects toward Cancer Cells.

Yu OuyangMichael FadeevPu ZhangRaanan CarmieliYang Sung SohnOla KarmiYunlong QinXinghua ChenRachel NechushtaiItamar Willner
Published in: ACS applied materials & interfaces (2022)
Aptamer-functionalized Ce 4+ -ion-modified C-dots act as catalytic hybrid systems, aptananozymes, catalyzing the H 2 O 2 oxidation of dopamine. A series of aptananozymes functionalized with different configurations of the dopamine binding aptamer, DBA, are introduced. All aptananozymes reveal substantially enhanced catalytic activities as compared to the separated Ce 4+ -ion-modified C-dots and aptamer constituents, and structure-catalytic functions between the structure and binding modes of the aptamers linked to the C-dots are demonstrated. The enhanced catalytic functions of the aptananozymes are attributed to the aptamer-induced concentration of the reaction substrates in spatial proximity to the Ce 4+ -ion-modified C-dots catalytic sites. The oxidation processes driven by the Ce 4+ -ion-modified C-dots involve the formation of reactive oxygen species ( • OH radicals). Accordingly, Ce 4+ -ion-modified C-dots with the AS1411 aptamer or MUC1 aptamer, recognizing specific biomarkers associated with cancer cells, are employed as targeted catalytic agents for chemodynamic treatment of cancer cells. Treatment of MDA-MB-231 breast cancer cells and MCF-10A epithelial breast cells, as control, with the AS1411 aptamer- or MUC1 aptamer-modified Ce 4+ -ion-modified C-dots reveals selective cytotoxicity toward the cancer cells. In vivo experiments reveal that the aptamer-functionalized nanoparticles inhibit MDA-MB-231 tumor growth.
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