Shewanella jiangmenensis sp. nov., isolated from aquaculture water.

A Gram-stain-negative and facultatively anaerobic bacterial strain designated as JM162201 T was isolated from aquaculture water for farming Pacific white shrimp (Litopenaeus vannamei). The genome size of strain JM162201 T was 4,436,316 bp, and the genomic DNA G + C content was 55.0%. Phylogenetic analysis based on 16S rRNA gene sequences and genomes showed that strain JM162201 T belonged to the genus Shewanella and was closely related to Shewanella litorisediminis SMK1-12 T (97.1%), Shewanella khirikhana TH2012 T (97.0%), and Shewanella amazonensis SB2B T (96.0%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain JM162201 T and three reference type strains were below the recognized thresholds of 95.0-96.0% (for ANI) and 70.0% (for dDDH) for species delineation. Growth occurred at 10-40 °C (optimum, 30 °C), at pH 4.0-10.0 (optimum, 7.0-8.0), and in 0-6.0% NaCl (w/v, optimum, 0-0.1%). The major cellular fatty acids of strain JM162201 T were summed feature 3 (C 16:1 ω7c and/or C 16:1 ω6c), C 17:1 ω8c, iso-C 15:0 , C 16:0 , and C 15:0 . The predominant quinones were MK7, Q-7, and Q-8. The major polar lipids were phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). Based on the polyphasic taxonomical analyses, strain JM162201 T represents a novel species of the genus Shewanella, for which the name Shewanella jiangmenensis sp. nov. is proposed, with the type strain JM162201 T (= GDMCC 1.2006 T  = KCTC 82340 T ).