Photo-regulated genetic encoding of dibenzo[ c,g ][1,2]diazocine on proteins via configuration switching.

Tingting ZhengJielin FuQin XiongXin ShenBaolin LiXiaohu ZhaoZhipeng Yu

Published in: Chemical communications (Cambridge, England) (2023)

We report two evolved Methanosarcina mazei pyrrolysine tRNA synthetases to genetically incorporate the isomers of dibenzo[ c,g ][1,2]diazocine-alanine (DBDAA) into proteins either in the dark or under regulation of 405 nm photo-stimulation. The genetic-encoded DBDAA realizes photo-tuning of enzymatic activity via the host-guest recognition of cucurbit[7]uril.

Keyphrases

genome wide
electron transfer
copy number
photodynamic therapy
transcription factor
hydrogen peroxide
dna methylation