"Off-pore" nucleoporins relocalize heterochromatic breaks through phase separation.

Chiara MeriglianoTaehyun RyuColby D SeeChristopher P CaridiXiao LiNadejda L ButovaTrevor W ReynoldsChangfeng DengDavid M ChenowethMaya CapelsonIrene E Chiolo

Published in: bioRxiv : the preprint server for biology (2024)

Nup88 and Sec13 recruit Nup98 to heterochromatic DSBs downstream from Smc5/6Nup88, Sec13 and Nup98 promote repair focus mobilization in heterochromatin 'off-pore'Nup98 excludes Rad51 from repair sites inside the heterochromatin domainPhase separation by Nup98 is required and sufficient for relocalization and Rad51 exclusion.

Keyphrases

dna damage
dna repair
oxidative stress
mass spectrometry