Inhibition of bacterial growth through LED (light-emitting diode) 465 and 630 nm: in vitro.

Photobiomodulation has been used to inactivate bacterial growth, in different laser or LED protocols. Thus, the aim of this study was to verify the inhibition of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli, in ATCC strains and bacteria collected from patients with skin burns, after irradiation with LED; 300 μl of saline solution with bacterial suspension was irradiated at a concentration of 0.5-0.63, by the McFarland scale, after five serial dilutions, with evaluation of pre- and post-irradiation pH and temperature control. The cultures were placed in a bacteriological incubator at 37 °C for 24 h for later counting of colony-forming units (CFU). Data were analyzed by Shapiro-Wilk tests and single-factor ANOVA, with Tukey post hoc (p < 0.05). Both wavelengths and energy densities tested showed inhibition of bacterial growth. The comparison of the irradiated groups (ATCC) with the control group showed the following: S. aureus and P. aeruginosa 465 nm (40 J/cm 2 ) and 630 nm (50 J/cm 2 ) and E. coli 465 nm (40 J/cm 2 ) and 630 nm (30 J/cm 2 ). Among the ATCC S. aureus groups, there was a difference for 630 nm (30 J/cm 2 ) and 465 nm (30, 40, 50 J/cm 2 ). The bacteria from the burned patients were S. aureus (30 and 50 J/cm 2 ) and P. aeruginosa (50 J/cm 2 ). We conclude that different bacterial strains were reduced into colony-forming units after LED irradiation.