Ratiometric fluorescence imaging of lysosomal NO in living cells and mice brains with Alzheimer's disease.
Rui HuangZiwei ZhangZhen ShiYumeng YangJunyong SunFeng GaoPublished in: Chemical communications (Cambridge, England) (2024)
We report an integrated ratiometric lysosomal nitric oxide (NO) nanoprobe based on engineered semiconducting polymer dots (Pdots), LyNO-Pdots, which consist of a newly designed NO-responsive dye, a fluorescent conjugated polymer and two functional polymers. The developed probe LyNO-Pdots exhibit high specificity and stability, good photostability and favorable blood-brain barrier (BBB) penetration ability. The LyNO-Pdots are successfully applied to ratiometric imaging of lysosomal NO variations in brain-derived endothelial cells, brain tissues and mice brains with Alzheimer's disease (AD). The results demonstrate that the NO content in the brains of AD mice is considerably higher than that in normal mice.
Keyphrases
- living cells
- fluorescent probe
- blood brain barrier
- fluorescence imaging
- high fat diet induced
- quantum dots
- single molecule
- nitric oxide
- endothelial cells
- high resolution
- photodynamic therapy
- cerebral ischemia
- hydrogen peroxide
- white matter
- cognitive decline
- sensitive detection
- gene expression
- wild type
- adipose tissue
- high glucose
- simultaneous determination
- visible light