Multiphoton single-molecule localization by sequential excitation with light minima.

Published in: Light, science & applications (2022)

Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1-2 nm precision with moderate photon counts, enabling tracking and super-resolution imaging with true molecular resolution. Expanding this concept to multi-photon regimes may be a useful complement to reach even higher localization precision and get deeper into biological specimens.

Keyphrases

single molecule
living cells
atomic force microscopy
energy transfer
high resolution
photodynamic therapy
high intensity
fluorescent probe
peripheral blood
mass spectrometry