Phosphorylated transcription factor PuHB40 mediates ROS-dependent anthocyanin biosynthesis in pear exposed to high light.
Lu ZhangLu WangYongchen FangYuhao GaoShulin YangJun SuJunbei NiYuanwen TengSongling BaiPublished in: The Plant cell (2024)
Plants are increasingly vulnerable to environmental stresses because of global warming and climate change. Stress-induced reactive oxygen species (ROS) accumulation results in plant cell damage and even cell death. Anthocyanins are important antioxidants that scavenge ROS to maintain ROS homeostasis. However, the mechanism underlying ROS-induced anthocyanin accumulation is unclear. In this study, we determined that the HD-Zip I family member transcription factor PuHB40 mediates ROS-dependent anthocyanin biosynthesis under high-light stress in pear (Pyrus ussuriensis). Specifically, PuHB40 induces the PuMYB123-like-PubHLH3 transcription factor complex for anthocyanin biosynthesis. PuHB40-mediated transcriptional activation depends on its phosphorylation level, which is regulated by protein phosphatase PP2A. Elevated ROS content maintains high PuHB40 phosphorylation levels, while also enhancing PuHB40-induced PuMYB123-like transcription by decreasing PuPP2AA2 expression, ultimately leading to increased anthocyanin biosynthesis. Our study reveals a pathway regulating ROS-induced anthocyanin biosynthesis in pear, further clarifying the mechanism underlying abiotic stress-induced anthocyanin biosynthesis, which may have implications for improving plant stress tolerance.
Keyphrases
- reactive oxygen species
- cell death
- stress induced
- transcription factor
- dna damage
- cell wall
- climate change
- diabetic rats
- high glucose
- oxidative stress
- gene expression
- cell cycle arrest
- stem cells
- bone marrow
- single cell
- cell proliferation
- heat stress
- small molecule
- risk assessment
- mesenchymal stem cells
- endothelial cells
- heat shock