Development of a New Solid-Phase Extraction Base Method for Free Saccharide Content Estimation of Meningococcal Conjugate Vaccines.
Riccardo De RiccoFrancesca RechValeria OnnisSalvatore Sanna CocconeGiusy ScaliaCristina MarcozziMassimiliano GaviniSara BeniSara GianniniLuca NompariChiara ParlatiClaudia MagagnoliSimona CianettiFrancesco BertiPublished in: ACS omega (2022)
GlaxoSmithKline (GSK) is currently developing a fully liquid presentation to ease the administration of the licensed quadrivalent conjugate vaccine (Menveo) against meningococcal serogroup A, C, W, and Y (MenACWY) infections. Herein, we report a new method for determining the free saccharide (FS) content of CRM 197 -MenACWY conjugated antigens, with the aim of improving accuracy and reproducibility. Mathematical models have been used to support technical knowledge in reducing the need for experimental development. This results in an improved, faster, and platform-based technique for FS separation with one single pretreatment applicable to all antigens of the multivalent meningococcal vaccine.
Keyphrases
- solid phase extraction
- liquid chromatography
- high performance liquid chromatography
- liquid chromatography tandem mass spectrometry
- molecularly imprinted
- dendritic cells
- simultaneous determination
- tandem mass spectrometry
- cancer therapy
- gas chromatography mass spectrometry
- mass spectrometry
- photodynamic therapy
- high throughput
- gas chromatography
- cell proliferation
- ultra high performance liquid chromatography
- ms ms
- high resolution