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Development of a real-time RT-PCR assay for the detection of pan-human parechoviruses.

Huanhuan LuJinbo XiaoKeyi ZhangZhenzhi HanYang SongDongyan WangTianjiao JiDongmei YanShuangli ZhuWenbo XuYanjun Zhang
Published in: Virology journal (2021)
Our findings suggested that the real-time RT-PCR assay developed in this study can be applied for routine PeV-A identification. We detected PeV-A1, 4 and 6 genotypes in the 728 faecal samples using this method. Additionally, we believe that our results will serve as a foundation for further studies on PeV-As and facilitate the expansion of the gene sequence information available in GenBank.
Keyphrases
  • real time pcr
  • high throughput
  • endothelial cells
  • clinical practice
  • genome wide
  • case control
  • transcription factor
  • genome wide identification