Astrovirus replication is dependent on induction of double membrane vesicles through a PI3K-dependent, LC3-independent pathway.
Theresa BubVirginia HargestShaoyuan TanMaria SmithAna Vazquez-PaganTim FlerlagePamela H BriglebVictoria A MeliopoulosBrett LindenbachValerie CortezJeremy Chase CrawfordStacey Schultz-CherryPublished in: bioRxiv : the preprint server for biology (2023)
These studies provide critical new evidence that astrovirus replication requires formation of double membrane vesicles, which utilize class III PI3K, but not LC3 conjugation autophagy machinery for biogenesis. These results are consistent with replication mechanisms for other positive sense RNA viruses. This suggests that targeting PI3K could be a promising therapeutic option for not only astrovirus, but other positive sense RNA virus infections.