HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples.

Samuel J Schulte Mark E FornaceJohn K HallNiles A Pierce

Published in: bioRxiv : the preprint server for biology (2023)

Spectral imaging with signal amplification based on the mechanism of hybridization chain reaction enables robust 10-plex, quantitative, high-resolution imaging of RNA and protein targets in whole-mount vertebrate embryos and brain sections.

Keyphrases

high resolution
mass spectrometry
optical coherence tomography
high speed
protein protein
computed tomography
resting state
amino acid
functional connectivity