The anti-fungal β-sitosterol targets the yeast oxysterol-binding protein Osh4.

Our results indicate that the concentrations of β-sitosterol, and Osh4, as well as its homologues within cells, are most likely the main determinants of β-sitosterol toxicity. Furthermore, some fungal species do not take up sterols, e.g. Saccharomyces cerevisiae, under aerobic conditions. Therefore, sterol uptake may also contribute to the β-sitosterol anti-fungal effect. These findings enable predicting the toxicity of β-sitosterol against plant fungal pathogens. © 2019 Society of Chemical Industry.