Hydrogen-deuterium exchange mass spectrometry highlights conformational changes induced by factor XI activation and binding of factor IX to factor XIa.

Awital Bar BarroetaJosse van GalenIngrid StrooJ Arnoud MarquartAlexander Benjamin Meijer Joost C M Meijers

Published in: Journal of thrombosis and haemostasis : JTH (2019)

We propose that the conformational changes of the catalytic domain upon activation increase the accessibility to the apple 3 domain to enable FIX binding. Moreover, our HDX MS results support the location of the proposed FIX binding site at the beginning of the apple 3 domain and suggest a mediating role in FIX binding for both loops adjacent to the apple 3 domain.

Keyphrases

mass spectrometry
molecular dynamics simulations
molecular dynamics
single molecule
multiple sclerosis
dna binding
ms ms
high resolution
high performance liquid chromatography