Identification of bifucosylated glycoforms using low-energy CID spectra.
András ÁcsLilla TuriákÁgnes RévészKároly VékeyLaszlo DrahosPublished in: Journal of mass spectrometry : JMS (2019)
We have used tandem mass spectrometry (MS/MS)-based analysis of glycopeptides in order to identify the composition and structure of rare glycoforms. The results illustrate utility of low-energy MS/MS for structure identification. We have shown the presence of bifucosylated and trifucosylated glycoforms in human α-1-acid glycoprotein (AGP), a major plasma glycoprotein. Fucosylation in the case of AGP always occurs on the antennae; core fucosylation was not observed.
Keyphrases
- ms ms
- tandem mass spectrometry
- ultra high performance liquid chromatography
- high performance liquid chromatography
- simultaneous determination
- liquid chromatography
- liquid chromatography tandem mass spectrometry
- gas chromatography
- solid phase extraction
- endothelial cells
- high resolution mass spectrometry
- bioinformatics analysis
- high resolution
- mass spectrometry
- induced pluripotent stem cells
- density functional theory