Simplified molecular diagnosis of visceral leishmaniasis: Laboratory evaluation of miniature direct-on-blood PCR nucleic acid lateral flow immunoassay.

Norbert J van DijkDawit Gebreegziabiher HagosDaniela M HugginsEugenia CarrilloSophia AjalaCarmen ChicharroDavid KiptanuiJose Carlos SolanaEdwin AbnerDawit WoldayHenk D F H Schallig

Published in: PLoS neglected tropical diseases (2024)

Mini-dbPCR-NALFIA is a promising tool for simplified molecular diagnosis of VL and follow-up of treated patients in blood samples. Future studies should evaluate its use in endemic, resource-limited settings, where mini-dbPCR-NALFIA may provide an accurate and versatile alternative to rK39 ICTs and aspirate microscopy.

Keyphrases

nucleic acid
newly diagnosed
single molecule
high resolution
prognostic factors
high throughput
current status
patient reported outcomes
optical coherence tomography
mass spectrometry
quantum dots
sensitive detection