CCA repair or ECA ligation-Which middle cerebral artery occlusion is better in the reperfusion mouse model?
Yue HuZhen-Hong YangFeng YanShuang-Feng HuangRong-Liang WangZi-Ping HanJun-Fen FanYang-Min ZhengPing LiuYu-Min LuoSi-Jie LiPublished in: Ibrain (2023)
A reliable animal model is essential for ischemic stroke research. The implications of the external carotid artery (ECA) transection or common carotid artery (CCA) ligation have been described. Thus, a modified animal model, the CCA-repair model, has been established, and studies have shown that the CCA-repair model has potential advantages over the CCA-ligation model. However, whether the CCA-repair model is superior to the ECA-ligation model remains unclear. Sixty male C57BL/6 mice were randomly assigned to establish the CCA-repair ( n = 34) or ECA-ligation ( n = 26) models. Cerebral blood flow before middle cerebral artery occlusion (MCAO), immediately after MCAO and reperfusion were monitored and the operation duration, postoperative body weight, and food intake within 7 days, and the number of intraoperative and postoperative deaths within 7 days were recorded in the two models. Modified neurological severity scores and Bederson (0-5) scores were used to evaluate postoperative neurological function deficits on Days 1/3/5/7. 2,3,5-Triphenyltetrazolium chloride staining was used to quantify lesion volume on Day 7 after the operation. We found the establishment of the CCA-repair model required a longer total operation duration ( p = 0.0175), especially the operation duration of reperfusion ( p < 0.0001). However, there was no significant difference in body weight and food intake development, lesion volume and intragroup variability, neurological function deficits, mortality, and survival probability between the two groups. The CCA-repair model has no significant advantage over the ECA-ligation model. The ECA-ligation model is still a better choice for focal cerebral ischemia.
Keyphrases
- cerebral ischemia
- middle cerebral artery
- body weight
- patients undergoing
- mouse model
- traumatic brain injury
- heart failure
- subarachnoid hemorrhage
- brain injury
- acute myocardial infarction
- blood brain barrier
- acute coronary syndrome
- climate change
- cardiovascular disease
- skeletal muscle
- risk factors
- cardiovascular events
- high fat diet induced