Mouse dendritic cells and other myeloid subtypes in healthy lymph nodes and skin: 26-Color flow cytometry panel for immune phenotyping.
Florian HornsteinerMartina M SykoraChristoph H TrippSieghart SopperPatrizia StoitznerPublished in: European journal of immunology (2022)
This novel 26-color flow cytometry panel allows the detailed immune phenotyping of the complex network of myeloid cells in murine lymph nodes and skin. With the optimized panel the different murine DC-subsets and other myeloid cell types can be identified and further characterized for co-stimulatory and inhibitory surface molecules.
Keyphrases
- flow cytometry
- dendritic cells
- lymph node
- regulatory t cells
- immune response
- high throughput
- induced apoptosis
- soft tissue
- single cell
- neoadjuvant chemotherapy
- sentinel lymph node
- wound healing
- acute myeloid leukemia
- cell cycle arrest
- cell therapy
- peripheral blood
- stem cells
- oxidative stress
- early stage
- cell proliferation
- signaling pathway