Functionalization of Biotinylated Polyethylene Glycol on Live Magnetotactic Bacteria Carriers for Improved Stealth Properties.
Richa ChaturvediYumin KangYunji EomSri Ramulu ToratiCheolGi KimPublished in: Biology (2021)
The early removal of drug delivery agents before reaching the affected target remains an area of interest to researchers. Several magnetotactic bacteria (MTB) have been used as self-propelled drug delivery agents, and they can also be controlled by an external magnetic field. By attaching the PEG-biotin polymer, the bacteria are turned into a stealth material that can escape from the phagocytosis process and reach the area of interest with the drug load. In the study, we developed a potential drug carrier by attaching the PEG-biotin to the MTB-through-NHS crosslinker to form a MTB/PEG-biotin complex. The attachment stability, efficacy, and bacterial viability upon attachment of the PEG-biotin polymer were investigated. Biological applications were carried out using a cytotoxicity assay of THP-1 cells, and the results indicate that the MTB/PEG-biotin complex is less harmful to cell viability compared to MTB alone. Along with cytotoxicity, an assay for cell association was also evaluated to assess the complex as a potential stealth material. The development of these complexes focuses on an easy, time-saving, and stable technique of polymer attachment with the bacteria, without damaging the cell's surface, so as to make it a strong and reliable delivery agent.
Keyphrases
- drug delivery
- mycobacterium tuberculosis
- pulmonary tuberculosis
- cancer therapy
- drug release
- single cell
- high throughput
- cell therapy
- induced apoptosis
- emergency department
- signaling pathway
- patient safety
- adverse drug
- endoplasmic reticulum stress
- mesenchymal stem cells
- risk assessment
- climate change
- cell death
- atomic force microscopy
- high speed