Pellino-2 in nonimmune cells: novel interaction partners and intracellular localization.
Ileana CristeaOve BrulandIngvild AukrustEyvind RødahlCecilie BredrupPublished in: FEBS letters (2021)
Pellino-2 is an E3 ubiquitin ligase that mediates intracellular signaling in innate immune pathways. Most studies of endogenous Pellino-2 have been performed in macrophages, but none in nonimmune cells. Using yeast two-hybrid screening and co-immunoprecipitation, we identified six novel interaction partners of Pellino-2, with various localizations: insulin receptor substrate 1, NIMA-related kinase 9, tumor necrosis factor receptor-associated factor 7, cyclin-F, roundabout homolog 1, and disheveled homolog 2. Pellino-2 showed cytoplasmic localization in a wide range of nonimmune cells under physiological potassium concentrations. Treatment with the potassium ionophore nigericin resulted in nuclear localization of Pellino-2, which was reversed by the potassium channel blocker tetraethylammonium. Live-cell imaging revealed intracellular migration of GFP-tagged Pellino-2. In summary, Pellino-2 interacts with proteins at different cellular locations, taking part in dynamic processes that change its intracellular localization influenced by potassium efflux.
Keyphrases
- induced apoptosis
- cell cycle arrest
- type diabetes
- reactive oxygen species
- innate immune
- rheumatoid arthritis
- high resolution
- signaling pathway
- mass spectrometry
- pi k akt
- oxidative stress
- adipose tissue
- cell cycle
- cell proliferation
- binding protein
- photodynamic therapy
- replacement therapy
- protein kinase
- amino acid
- drug induced
- combination therapy
- fluorescence imaging
- saccharomyces cerevisiae
- angiotensin converting enzyme