Microglial cell-derived interleukin-6 influences behavior and inflammatory response in the brain following traumatic brain injury.
Paula SanchisOlaya Fernández-GayolJoel VizuetaGemma ComesCarla CanalAnna EscrigAmalia MolineroMercedes GiraltJuan HidalgoPublished in: Glia (2019)
Traumatic brain injury (TBI) is a major health problem with high rates of mortality and morbidity worldwide. The response of the brain to TBI is orchestrated by a number of cytokines, including interleukin-6 (IL-6). IL-6 is a major cytokine in the central nervous system and it is produced by different cells, such as neurons, glial cells, and endothelial cells. Since glial cells are one of the most important sources and targets of IL-6, we have examined the role of microglia-derived IL-6 in normal conditions and following a model of TBI, cryolesion of the somatosensorial cortex. To this end, tamoxifen-inducible microglial IL-6-deficient (Il6ΔMic , using Cx3cr1 CreER model) mice and control (Il6lox/lox ) mice were used. In normal conditions, microglial IL-6 deficiency reduced deambulation and exploratory behavior and decreased anxiety in a sex-dependent manner. The transcriptome profile following cryolesion was dramatically altered 1 day post-lesion in Il6ΔMic compared with Il6lox/lox mice. However, the phenotype of Il6ΔMic mice was less compromised in the following days, suggesting that compensatory mechanisms are at play.
Keyphrases
- traumatic brain injury
- inflammatory response
- induced apoptosis
- endothelial cells
- healthcare
- lipopolysaccharide induced
- mental health
- public health
- high fat diet induced
- insulin resistance
- physical activity
- multiple sclerosis
- gene expression
- spinal cord
- oxidative stress
- risk factors
- white matter
- lps induced
- coronary artery disease
- risk assessment
- single cell
- genome wide
- endoplasmic reticulum stress
- functional connectivity
- sleep quality
- cerebral ischemia