Detection limit of 89Zr-labeled T cells for cellular tracking: an in vitro imaging approach using clinical PET/CT and PET/MRI.
Laura M LechermannRoido ManavakiBala AttiliDoreen LauLorna B JarvisTim D FryerNick BirdLuigi AlojNeel PatelBristi BasuMatthew ClevelandFranklin I AigbirhioJoanne L JonesFerdia A GallagherPublished in: EJNMMI research (2020)
The results show the feasibility of detecting [89Zr]Zr(oxinate)4-labeled Jurkat T cells on clinical PET systems. The results provide a best-case scenario, as in vivo detection using PET/CT or PET/MRI will be affected by cell number, specific activity per cell, the density of cells within the target volume, and non-specific signal. This work has important implications for cell labeling studies in patients, particularly when using radiosensitive cells (e.g., T cells), which require detection of low cell numbers while minimizing radiation dose per cell.
Keyphrases
- pet ct
- pet imaging
- single cell
- positron emission tomography
- cell therapy
- computed tomography
- induced apoptosis
- magnetic resonance imaging
- stem cells
- end stage renal disease
- oxidative stress
- high resolution
- mesenchymal stem cells
- bone marrow
- chronic kidney disease
- cell cycle arrest
- loop mediated isothermal amplification
- contrast enhanced
- cell proliferation
- mass spectrometry
- real time pcr
- label free
- signaling pathway
- prognostic factors
- peritoneal dialysis
- patient reported