Development of an LC-MS/MS assay for quantification of intact INSL3 in rat plasma.
Nicole A SchneckLela MortezaviAlan R OlzinskiDiane PosavecLarry J JolivetteTimothy W SikorskiShan-Shan ZhangChristine G SchnackenbergHermes Licea-PerezPublished in: Bioanalysis (2023)
Background: Relatively large disulfide-linked polypeptides can serve as signaling molecules for a diverse array of biological processes and may be studied in animal models to investigate their function in vivo . The aim of this work was to develop an LC-MS/MS assay to measure a model peptide, INSL3, in rat plasma. Results: A dual enrichment strategy incorporating both protein precipitation and solid phase extraction was utilized to isolate INSL3 from rat plasma, followed by targeted LC-MS/MS detection. The method was able to measure full-length INSL3 (6.1 kDa) down to 0.2 ng/ml with acceptable accuracy and precision. Conclusion: The final assay was applied to support an exploratory pharmacokinetic study to evaluate steady-state concentrations of dosed INSL3 in rat plasma.
Keyphrases
- high throughput
- solid phase extraction
- liquid chromatography tandem mass spectrometry
- molecularly imprinted
- high performance liquid chromatography
- liquid chromatography
- high resolution
- mass spectrometry
- cancer therapy
- gas chromatography
- small molecule
- loop mediated isothermal amplification
- drug delivery
- amino acid
- sensitive detection