Cardioprotective Effects of Astragalin against Myocardial Ischemia/Reperfusion Injury in Isolated Rat Heart.
Daoxu QuJichun HanHuanhuan RenWenxiao YangXinjie ZhangQiusheng ZhengDong WangPublished in: Oxidative medicine and cellular longevity (2015)
This study aims to evaluate the cardioprotective effects of astragalin against myocardial ischemia/reperfusion (I/R) injury in isolated rat heart. The cardioprotective effects of astragalin on myocardial I/R injury were investigated on Langendorff apparatus. Adult male Sprague-Dawley rats were randomly divided into five groups. The results showed that astragalin pretreatment improved myocardial function. Compared with I/R group, lactate dehydrogenase (LDH) and creatine kinase (CK) activities in coronary flow decreased in astragalin pretreatment groups, whereas superoxide dismutase (SOD) activity and glutathione/glutathione disulfide (GSH/GSSG) ratio significantly increased. The levels of malondialdehyde (MDA), intracellular reactive oxygen species (ROS), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) decreased in astragalin-treated groups. The infarct size (IS) and apoptosis rate in hearts from astragalin-treated groups were lower than those in hearts from the I/R group. Western blot analysis also revealed that astragalin preconditioning significantly reduced Bax level, whereas Bcl-2 was increased in the myocardium. Therefore, astragalin exhibited cardioprotective effects via its antioxidative, antiapoptotic, and anti-inflammatory activities.
Keyphrases
- reactive oxygen species
- left ventricular
- anti inflammatory
- oxidative stress
- heart failure
- rheumatoid arthritis
- coronary artery
- south africa
- dna damage
- acute myocardial infarction
- atrial fibrillation
- brain injury
- mass spectrometry
- hydrogen peroxide
- young adults
- subarachnoid hemorrhage
- tyrosine kinase
- childhood cancer