Rhynchosia volubilis Promotes Cell Survival via cAMP-PKA/ERK-CREB Pathway.
Sang-Hyun AhnJung-Soo SuhYoon-Kwan JangHeon-Su KimGyu-Ho ChoiEunhye KimTae-Jin KimPublished in: Pharmaceuticals (Basel, Switzerland) (2022)
Rhynchosia volubilis , a small black bean, has been used as a traditional remedy to treat diseases and maintain health in East Asia, but its cellular effects and molecular mechanisms are not fully understood. The purpose of this study was to investigate the effect of ethanol extract from Rhynchosia volubilis (EERV) on cell survival and to elucidate the biochemical signaling pathways. Our results showed that EERV stimulated the cyclic AMP (cAMP) signal revealed by a fluorescent protein (FP)-based intensiometric sensor. Using a Förster resonance energy transfer (FRET)-based sensor, we further revealed that EERV could activate PKA and ERK signals, which are downstream effectors of cAMP. In addition, we reported that EERV could induce the phosphorylation of CREB, a key signal for cell survival. Thus, our results suggested that EERV protects against apoptosis by activating the cell survival pathway through the cAMP-PKA/ERK-CREB pathway.
Keyphrases
- energy transfer
- signaling pathway
- quantum dots
- protein kinase
- pi k akt
- binding protein
- cell proliferation
- oxidative stress
- cell cycle arrest
- healthcare
- epithelial mesenchymal transition
- induced apoptosis
- mental health
- cell death
- endoplasmic reticulum stress
- risk assessment
- health information
- amino acid
- label free
- type iii