SeedEZ™ Interdigitated Electrodes and Multifunctional Layered Biosensor Composites (MLBCs): A Paradigm Shift in the Development of In Vitro BioMicrosystems.
Charles M DidierAvra KunduJames T ShoemakerJelena VukasinovicSwaminathan RajaramanPublished in: Journal of microelectromechanical systems : a joint IEEE and ASME publication on microstructures, microactuators, microsensors, and microsystems (2020)
We have developed a new technology for the realization of composite biosensor systems, capable of measuring electrical and electrophysiological signals from electrogenic cells, using SeedEZ™ 3D cell culture-scaffold material. This represents a paradigm-shift for BioMEMS processing; 'Biology-Microfabrication' versus the standard 'Microfabrication-Biology' approach. An Interdigitated Electrode (IDE) developed on the 3D cell-scaffold was used to successfully monitor acute cardiomyocyte growth and controlled population decline. We have further characterized processability of the 3D scaffold, demonstrated long-term biocompatibility of the scaffold with various cell lines and developed a multifunctional layered biosensor composites (MLBCs) using SeedEZ™ and other biocompatible substrates for future multilayer sensor integration.
Keyphrases
- reduced graphene oxide
- tissue engineering
- gold nanoparticles
- sensitive detection
- quantum dots
- drug delivery
- label free
- induced apoptosis
- cancer therapy
- highly efficient
- cell cycle arrest
- cell therapy
- stem cells
- intensive care unit
- oxidative stress
- signaling pathway
- mesenchymal stem cells
- aqueous solution
- ion batteries