A High-Sensitivity 10-Color Flow Cytometric Minimal Residual Disease Assay in B-Lymphoblastic Leukemia/Lymphoma Can Easily Achieve the Sensitivity of 2-in-106 and Is Superior to Standard Minimal Residual Disease Assay: A Study of 622 Patients.
Prashant Ramesh TembharePapagudi G Subramanian PgSitaram GhogaleGaurav ChatterjeeNikhil V PatkarAvinash GuptaRahul ShuklaYajamanam BadrinathNilesh DeshpandeGaurav NarulaPearl RodriguesKarishma GiraseDilshad DhaliwalMaya PrasadDhanalaxmi ShettyShripad BanavaliSumeet GujralPublished in: Cytometry. Part B, Clinical cytometry (2019)
We report an easily reproducible high-sensitivity 10-color FC-MRD assay with the sensitivity of 2-in-106 (0.0002%). It allowed the detection of low-level MRD in samples, which could have been reported negative using the standard FC-MRD with limited event analysis. Thus, this high-sensitivity MRD-methodology can provide a reliable basis for therapeutic modifications in B-ALL. © 2019 International Clinical Cytometry Society.