Coculture of a Pathogenic Actinomycete and Animal Cells To Produce Nocarjamide, a Cyclic Nonapeptide with Wnt Signal-Activating Effect.

Yasumasa Hara Midori A Arai Kazufumi ToumeHyuma MasuTomoyuki SatoKatsuko KomatsuTakashi YaguchiMasami Ishibashi

Published in: Organic letters (2018)

A coculture method with a pathogenic actinomycete of the genus Nocardia and an animal cell line was designed to reconstruct and emulate the initial infection state, and a new cyclic nonapeptide, named nocarjamide (1), was obtained by coculture of Nocardia tenerifensis IFM 10554T and the mouse macrophage-like cell line J774.1 in a modified Czapek-Dox medium. Nocarjamide (1) exhibited Wnt signal-activating effects.

Keyphrases

signaling pathway
induced apoptosis
cell proliferation
stem cells
cell cycle arrest
adipose tissue
endoplasmic reticulum stress
pi k akt