Use of Purslane ( Portulaca oleracea ) Extracts and Cysteine in Diluents to Improve the Cryopreserved Quality of Goat Sperm.

Highlights Using cysteine and purslane extracts in extenders improved significantly the post-thaw sperm characteristics. Sperm viability, DNA integrity, and mitochondrial activity demonstrate an improvement in post-thaw sperm. Malondialdehyde production was decreased based on the positive effects of treated extenders. The obtained results demonstrate that supplementation of 50 μg/mL of purslane methanolic extract with cysteine to freezing extenders was significantly superior compared with other treatments.