Mitochondrial Targeting Long-Term Near-Infrared Imaging and Photodynamic Therapy Aggregation-Induced Emission Luminogens Manipulated by Thiophene.

In recent years, the development and application of integrated probes for theranostics have attracted more and more attention. However, few biological probes can meet the needs of in vivo and in vitro long-term near-infrared imaging and photodynamic therapy, especially with a certain subcellular organelle targeting ability. Here, 2-chlorophenothiazine as a pharmacophore is linked to the mitochondrial targeting group pyridine cation through an alkyl chain, which is further linked to triphenylamine-based aggregation-induced emission groups to obtain two aggregation-induced emission luminogens (AIEgens). Only the presence or absence of thiophene causes two AIEgens to exhibit different structure-oriented characteristics. Although they are different with respec to mitochondrial targeting, cellular imaging, and cytotoxicity, they all have excellent in vivo and in vitro long-term near-infrared imaging and photodynamic therapy capabilities.