Real-Time In Vivo Detection of Cellular Senescence through the Controlled Release of the NIR Fluorescent Dye Nile Blue.
Beatriz Lozano-TorresJuan F BlandezIrene GalianaAlba García-FernándezMaría AlfonsoMaría D MarcosMar OrzáezFélix SancenónRamón Martínez-MáñezPublished in: Angewandte Chemie (International ed. in English) (2020)
In vivo detection of cellular senescence is accomplished by using mesoporous silica nanoparticles loaded with the NIR-FDA approved Nile blue (NB) dye and capped with a galactohexasaccharide (S3). NB emission at 672 nm is highly quenched inside S3, yet a remarkable emission enhancement is observed upon cap hydrolysis in the presence of β-galactosidase and dye release. The efficacy of the probe to detect cellular senescence is tested in vitro in melanoma SK-Mel-103 and breast cancer 4T1 cells and in vivo in palbociclib-treated BALB/cByJ mice bearing breast cancer tumor.
Keyphrases
- photodynamic therapy
- dna damage
- endothelial cells
- label free
- living cells
- highly efficient
- fluorescent probe
- quantum dots
- stress induced
- loop mediated isothermal amplification
- drug delivery
- real time pcr
- fluorescence imaging
- light emitting
- drug release
- oxidative stress
- type diabetes
- aqueous solution
- solid state
- cancer therapy
- insulin resistance
- sensitive detection
- high fat diet induced
- skin cancer
- newly diagnosed
- single molecule