Preclinical Evaluation of NHS-Activated Gold Nanoparticles Functionalized with Bombesin or Neurotensin-Like Peptides for Targeting Colon and Prostate Tumours.
Livia Elena ChilugDana NiculaeRadu Anton LeonteAlexandrina NanRodica Paula TurcuCosmin MustaciosuRadu Marian SerbanVasile LavricGina MandaPublished in: Molecules (Basel, Switzerland) (2020)
Recent advances and large-scale use of hybrid imaging modalities like PET-CT have led to the necessity of improving nano-drug carriers that can facilitate both functional and metabolic screening in nuclear medicine applications. In this study, we focused on the evaluation of four potential imaging nanoparticle structures labelled with the 68Ga positron emitter. For this purpose, we functionalized NHS-activated PEG-gold nanoparticles with 68Ga-DOTA-Neuromedin B, 68Ga-DOTA-PEG(4)-BBN(7-14), 68Ga-DOTA-NT and 68Ga-DOTA-Neuromedin N. In vitro binding kinetics and specific binding to human HT-29 colon carcinoma cells and DU-145 prostate carcinoma cells respectively were assessed, over 75% retention being obtained in the case of 68Ga-DOTA-PEG(4)-BBN(7-14)-AuNP in prostate tumour cells and over 50% in colon carcinoma cells. Biodistribution in NU/J mice highlighted a three-fold uptake increase in tumours at 30 min post-injection of 68Ga-DOTA-NT-AuNP and 68Ga-DOTA-PEG(4)-BBN(7-14)-AuNP compared to 68Ga-DOTA-NT and 68Ga-DOTA-PEG(4)-BBN(7-14) respectively, therewith fast distribution in prostate and colon tumours and minimum accumulation in non-targeted tissues.
Keyphrases
- pet ct
- gold nanoparticles
- prostate cancer
- positron emission tomography
- drug delivery
- high resolution
- benign prostatic hyperplasia
- emergency department
- type diabetes
- stem cells
- endothelial cells
- induced apoptosis
- risk assessment
- oxidative stress
- patient safety
- computed tomography
- signaling pathway
- adipose tissue
- ultrasound guided
- binding protein
- high fat diet induced
- amino acid
- reduced graphene oxide