Transcriptome analysis in LRRK2 and idiopathic Parkinson's disease at different glucose levels.
Rubén Fernández-SantiagoAnna Esteve-CodinaManel FernándezFrancesc ValldeoriolaAlmudena Sanchez-GómezEsteban MuñozYaroslau ComptaEduardo TolosaMario EzquerraMaría J MartíPublished in: NPJ Parkinson's disease (2021)
Type-2 diabetes (T2D) and glucose metabolic imbalances have been linked to neurodegenerative diseases, including Parkinson's disease (PD). To detect potential effects of different glucose levels on gene expression, by RNA-seq we analyzed the transcriptome of dermal fibroblasts from idiopathic PD (iPD) patients, LRRK2-associated PD (L2PD) patients, and healthy controls (total n = 21 cell lines), which were cultured at two different glucose concentrations (25 and 5 mM glucose). In PD patients we identified differentially expressed genes (DEGs) that were related to biological processes mainly involving the plasmatic cell membrane, the extracellular matrix, and also neuronal functions. Such pathway deregulation was largely similar in iPD or L2PD fibroblasts. Overall, the gene expression changes detected in this study were associated with PD independently of glucose concentration.
Keyphrases
- gene expression
- end stage renal disease
- type diabetes
- extracellular matrix
- rna seq
- newly diagnosed
- ejection fraction
- chronic kidney disease
- blood glucose
- peritoneal dialysis
- metabolic syndrome
- endothelial cells
- patient reported outcomes
- brain injury
- blood brain barrier
- weight loss
- transcription factor
- patient reported
- human health