Biodistribution and intestinal inflammatory response following voluntary oral intake of silver nanoparticles by C57BL/6J mice.
Adelaide SousaRui AzevedoVera Marisa CostaSara OliveiraInês PreguiçaSofia VianaFlávio ReisAgostinho AlmeidaPaulo MatafomePatrícia Dias-PereiraFélix CarvalhoEduarda FernandesMarisa FreitasPublished in: Archives of toxicology (2023)
Silver nanoparticles (AgNP) are among the most widely commercialized nanomaterials globally, with applications in medicine and the food industry. Consequently, the increased use of AgNP in the food industry has led to an unavoidable rise in human exposure to these nanoparticles. Their widespread use raises concerns about potential hazards to human health, specifically their intestinal pro-inflammatory effects. Thus, the main objective of this study was to evaluate the biological effects of two subacute doses of 5 nm polyvinylpyrrolidone (PVP)-AgNP in C57BL/6J mice. One mg/kg body weight or 10 mg/kg bw was provided once a day for 14 days, using a new technology (HaPILLness) that allows voluntary, stress-free, and accurate oral dosing. It was observed that after oral ingestion, while AgNP is biodistributed throughout the entire organism, most of the ingested dose is excreted in the feces. The passage and accumulation of AgNP throughout the intestine instigated a prominent inflammatory response, marked by significant histological, vascular, and cellular transformations. This response was driven by the activation of the nuclear factor-кB (NF-кB) inflammatory pathway, ultimately leading to the generation of multiple cytokines and chemokines.
Keyphrases
- silver nanoparticles
- human health
- inflammatory response
- nuclear factor
- risk assessment
- toll like receptor
- body weight
- lps induced
- climate change
- lipopolysaccharide induced
- endothelial cells
- high fat diet induced
- oxidative stress
- signaling pathway
- type diabetes
- immune response
- metabolic syndrome
- high resolution
- insulin resistance
- pi k akt
- wild type
- stress induced