Detection of Sulfatase Enzyme Activity with a CatalyCEST MRI Contrast Agent.

Sanhita Sinharay Gabriela Fernández-CuervoJasmine P AcfalleMark D Pagel

Published in: Chemistry (Weinheim an der Bergstrasse, Germany) (2016)

A chemical exchange saturation transfer (CEST) MRI contrast agent has been developed that detects sulfatase enzyme activity. The agent produces a CEST signal at δ=5.0 ppm before enzyme activity, and a second CEST signal appears at δ=9.0 ppm after the enzyme cleaves a sulfate group from the agent. The comparison of the two signals improved detection of sulfatase activity.

Keyphrases

contrast enhanced
magnetic resonance imaging
magnetic resonance
loop mediated isothermal amplification
diffusion weighted imaging
computed tomography
real time pcr
sensitive detection