Evolutionarily conserved and testis-specific gene, 4930524B15Rik, is not essential for mouse spermatogenesis and fertility.
Ranjha KhanJingwei YeAyesha YousafWasim ShahAyesha AftabBasit ShahQamar ZamanMuhammad ZubairQinghua ShiXiaohua JiangPublished in: Molecular biology reports (2020)
Thousands of genes are involved in spermatogenesis, however, the functional roles of most these genes for male fertility remain to be discovered. This research focused to explore the function of evolutionarily conserved and testis-specific expressed gene 4930524B15Rik, which is known as C5orf47 in human. We generated 4930524B15Rik knockout mice by CRISPR/Cas9 technology and found 4930524B15Rik-/- mice were fertile. Furthermore, no averted abnormalities were observed in testis morphology, epididymal sperm contents and sperm morphology in 4930524B15Rik knockout mice. Subsequently, histological analysis of testicular tissue revealed intact structure of seminiferous tubules along with the presence of all types of germ cells in 4930524B15Rik-/- mice similar to wild type. Additionally, cytological analysis of spermatocytes displayed no significant differences in the prophase I progression of meiosis, further indicating that 4930524B15Rik have no essential function in mammalian spermatogenesis. Altogether, these results indicated that 4930524B15Rik is dispensable for fertility of male mice and these findings will help researchers to avoid future research overlap and to focus on genes that are crucial for spermatogenesis and reproduction.
Keyphrases
- genome wide
- genome wide identification
- wild type
- crispr cas
- transcription factor
- endothelial cells
- high fat diet induced
- induced apoptosis
- genome wide analysis
- dna methylation
- type diabetes
- bioinformatics analysis
- oxidative stress
- childhood cancer
- adipose tissue
- metabolic syndrome
- cell death
- cell cycle arrest
- insulin resistance
- skeletal muscle
- signaling pathway
- gene expression
- pi k akt