Development of an Affordable ELISA Targeting the SARS-CoV-2 Nucleocapsid and Its Application to Samples from the Ongoing COVID-19 Epidemic in Ghana.

Kesego TapelaPrecious C OpurumFranklin Y NuokpemBecky TettehGodfred K SiawMaria V HumbertSylvia Tawiah-EshunAnna Ibrahim BarakisuKwame AsieduSamuel Kojo ArhinAaron A ManuSekyibea N A Appiedu-AddoLouisa ObbengDarius QuansahSylvester LanguonClaudia AnyigbaDaniel DosooNelson K O EduDaniel Oduro-MensahWilliam AmpofoEmmanuel TagoeOsbourne QuayeIrene Owusu DonkorJewelna AkorliYaw AniwehMyron ChristodoulidesJoe MutungiYaw BediakoJulian C RaynerGordon A AwandareChristopher J McCormickPeter Kojo Quashie

Published in: Molecular diagnosis & therapy (2023)

This study developed a low-cost and transferable assay that enables highly sensitive and specific detection of human anti-SARS-CoV-2 IgG antibodies. This assay can be modified to include additional antigens and used for continuous monitoring of sero-exposure to SARS-CoV-2 in West Africa.

Keyphrases

sars cov
respiratory syndrome coronavirus
low cost
high throughput
endothelial cells
coronavirus disease
dendritic cells
label free
induced pluripotent stem cells
cancer therapy
drug delivery
fluorescent probe
living cells
real time pcr