Imaging flow cytometric detection of del(17p) in bone marrow and circulating plasma cells in multiple myeloma.
Thomas I MinchertonStephanie J LamSarah E ClarkeHenry Y L HuiJacques A J MalherbeHun S ChuahM Hasib SidiqiKathy A FullerWendy N ErberPublished in: International journal of laboratory hematology (2024)
The "immuno-flowFISH" imaging flow cytometric method could detect del(17p) in plasma cells in both bone marrow and blood samples of myeloma patients. This method was also able to detect gains and losses of chromosome 17, which are also of prognostic significance. The lowest levels of 0.009% (bone marrow) and 0.001% (blood) for chromosome 17 abnormalities was below the detection limit of current FISH method. This method offers potential as a new means of identifying these prognostically important chromosomal defects, even when only rare cells are present and for serial disease monitoring.
Keyphrases
- bone marrow
- induced apoptosis
- cell cycle arrest
- mesenchymal stem cells
- multiple myeloma
- high resolution
- newly diagnosed
- end stage renal disease
- endoplasmic reticulum stress
- copy number
- cell death
- oxidative stress
- chronic kidney disease
- signaling pathway
- dna methylation
- prognostic factors
- cell proliferation
- mass spectrometry
- risk assessment
- peritoneal dialysis
- patient reported outcomes
- photodynamic therapy
- fluorescence imaging
- genome wide