Cytotoxicity of Different Concentrations of Three Root Canal Sealers on Human Mesenchymal Stem Cells.
Shankargouda PatilReem Al AjlanHala MitwalliNour AburaisiAmer MahmoodManikandan MuthuranganRanda AlmadhriMusaad AlfayezSukumaran AnilPublished in: Biomolecules (2018)
This study assessed the dose-dependent effect on the cytotoxicity of BioRoot RCS (BR) and Endosequence BC (BC) sealers in human bone marrow mesenchymal stem cells (hMSCs) compared to those of the AH Plus sealer. Cells were exposed to different dilutions of extracts from freshly prepared sealers (1:2, 1:8, 1:32). Unexposed cells acted as the negative control. Cytotoxicity was evaluated by an alamar blue assay. Cell morphology was analyzed by using scanning electron microscopy after exposure to the different sealers' extracts. Statistical analysis was performed using a one-way analysis of variance and the Bonferroni post hoc test (p < 0.05). The cytotoxicities of BC and BR were less than that of AH Plus. In the presence of 1:2 BR, the cell proliferation was significantly lower than the control. At 1:8 and 1:32 concentrations, both the tricalcium silicate sealers led to similar cellular proliferation. Cells in BC and BR sealers' extracts spread better than those in AH Plus extract.
Keyphrases
- induced apoptosis
- cell proliferation
- cell cycle arrest
- electron microscopy
- mesenchymal stem cells
- endothelial cells
- oxidative stress
- signaling pathway
- endoplasmic reticulum stress
- cell death
- single cell
- high throughput
- bone marrow
- cell therapy
- cell cycle
- mass spectrometry
- anti inflammatory
- light emitting
- bone regeneration