An Aptamer against MNK1 for Non-Small Cell Lung Cancer Treatment.
Rebeca Carrión-MarchanteCelia Pinto-DíezJosé Ignacio Klett-MingoEsther PalaciosMiriam Barragán-UseroM Isabel Pérez-MorgadoManuel Pascual-MelladoSonia AlcaláLaura Ruiz-CañasBruno SainzVíctor M GonzálezMaría Elena MartínPublished in: Pharmaceutics (2023)
Lung cancer is the leading cause of cancer-related death worldwide. Its late diagnosis and consequently poor survival make necessary the search for new therapeutic targets. The mitogen-activated protein kinase (MAPK)-interacting kinase 1 (MNK1) is overexpressed in lung cancer and correlates with poor overall survival in non-small cell lung cancer (NSCLC) patients. The previously identified and optimized aptamer from our laboratory against MNK1, apMNKQ2, showed promising results as an antitumor drug in breast cancer in vitro and in vivo. Thus, the present study shows the antitumor potential of apMNKQ2 in another type of cancer where MNK1 plays a significant role, such as NSCLC. The effect of apMNKQ2 in lung cancer was studied with viability, toxicity, clonogenic, migration, invasion, and in vivo efficacy assays. Our results show that apMNKQ2 arrests the cell cycle and reduces viability, colony formation, migration, invasion, and epithelial-mesenchymal transition (EMT) processes in NSCLC cells. In addition, apMNKQ2 reduces tumor growth in an A549-cell line NSCLC xenograft model. In summary, targeting MNK1 with a specific aptamer may provide an innovative strategy for lung cancer treatment.
Keyphrases
- small cell lung cancer
- cell cycle
- epithelial mesenchymal transition
- advanced non small cell lung cancer
- gold nanoparticles
- sensitive detection
- end stage renal disease
- oxidative stress
- brain metastases
- signaling pathway
- protein kinase
- cell migration
- cell proliferation
- induced apoptosis
- newly diagnosed
- magnetic nanoparticles
- ejection fraction
- transforming growth factor
- tyrosine kinase
- peritoneal dialysis
- epidermal growth factor receptor
- chronic kidney disease
- cell therapy
- free survival
- squamous cell carcinoma
- cell cycle arrest
- drug delivery
- endoplasmic reticulum stress
- label free
- high throughput
- mesenchymal stem cells
- pi k akt
- squamous cell
- cell death
- bone marrow