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Scaling up of a Self-Confined Catalytic Hybridization Circuit for Robust microRNA Imaging.

Xue GongRuomeng LiJiajia ZhangPu ZhangZhongwei JiangLianzhe HuXiaoqing LiuYi WangFuan Wang
Published in: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2024)
The precise regulation of cellular behaviors within a confined, crowded intracellular environment is highly amenable in diagnostics and therapeutics. While synthetic circuitry system through a concatenated chemical reaction network has rarely been reported to mimic dynamic self-assembly system. Herein, a catalytic self-defined circuit (CSC) for the hierarchically concatenated assembly of DNA domino nanostructures is engineered. By incorporating pre-sealed symmetrical fragments into the preying hairpin reactants, the CSC system allows the hierarchical DNA self-assembly via a microRNA (miRNA)-powered self-sorting catalytic hybridization reaction. With minimal strand complexity, this self-sustainable CSC system streamlined the circuit component and achieved localization-intensified cascaded signal amplification. Profiting from the self-adaptively concatenated hybridization reaction, a reliable and robust method has been achieved for discriminating carcinoma tissues from the corresponding para-carcinoma tissues. The CSC-sustained self-assembly strategy provides a comprehensive and smart toolbox for organizing various hierarchical DNA nanostructures, which may facilitate more insights for clinical diagnosis and therapeutic assessment.
Keyphrases
  • nucleic acid
  • single molecule
  • circulating tumor
  • cell free
  • gene expression
  • high resolution
  • label free
  • crystal structure
  • electron transfer
  • circulating tumor cells
  • fluorescence imaging