Detection and Quantification of UV-irradiation-induced DNA Damages by Liquid Chromatography-Mass Spectrometry and Immunoassay†.
Weiyi LaiHai-Lin WangPublished in: Photochemistry and photobiology (2021)
Solar ultraviolet (UV)-induced DNA lesions are associated with skin carcinogenesis. The detection of these DNA lesions is important to understand their genotoxicity and health effects. However, sunlight exposure-relevant DNA damage measurement is still a challenge. Here, we summarize our recent progresses on the related analytical techniques, including synthesis of dimeric lesions, the optimization of procedures for ultrahigh performance liquid chromatography-coupled mass spectrometry (UHPLC-MS/MS), and the maturation of anti-T(6-4)T photoproduct antibodies and their potential applications for immunoassay.
Keyphrases
- liquid chromatography
- mass spectrometry
- high resolution mass spectrometry
- tandem mass spectrometry
- ms ms
- ultra high performance liquid chromatography
- circulating tumor
- simultaneous determination
- dna damage
- high performance liquid chromatography
- label free
- cell free
- single molecule
- gas chromatography
- solid phase extraction
- high glucose
- diabetic rats
- liquid chromatography tandem mass spectrometry
- capillary electrophoresis
- high resolution
- loop mediated isothermal amplification
- oxidative stress
- real time pcr
- sensitive detection
- radiation therapy
- dna repair
- endothelial cells