Polarimetric second-harmonic generation microscopy of the hierarchical structure of collagen in stage I-III non-small cell lung carcinoma.
Ahmad GolaraeiLeila B Mostaço-GuidolinVaishnavi RajaRoya NavabTao WangShingo SakashitaKazuhiro YasufukuMing-Sound TsaoBrian C WilsonVirginijus BarzdaPublished in: Biomedical optics express (2020)
Polarimetric second-harmonic generation (P-SHG) microscopy is used to quantify the structural alteration of collagen in stage-I,-II and -III non-small cell lung carcinoma (NSCLC) ex vivo tissue. The achiral and chiral molecular second-order susceptibility tensor components ratios (R and C, respectively), the degree of linear polarization (DLP) and the in-plane collagen fiber orientation (δ) were extracted. Further, texture analysis was performed on the SHG intensity, R, C, DLP and δ. The distributions of R, C, DLP and δ as well as the textural features of entropy, correlation and contrast show significant differences between normal and tumor tissues.
Keyphrases
- single molecule
- single cell
- high resolution
- cell therapy
- small cell lung cancer
- wound healing
- magnetic resonance
- gene expression
- high speed
- tissue engineering
- contrast enhanced
- high intensity
- stem cells
- magnetic resonance imaging
- label free
- advanced non small cell lung cancer
- tyrosine kinase
- capillary electrophoresis