A DNAzyme-mediated target-initiated rolling circle amplification strategy based on a microchip platform for the detection of apurinic/apyrimidinic endonuclease 1 at the single-cell level.

Chunhuan XuShengyu ChenJingjin ZhaoXiaoshu LuoShu-Lin Zhao

Published in: Chemical communications (Cambridge, England) (2021)

A DNAzyme-mediated target-initiated rolling circle signal amplification strategy based on a microchip platform was developed for detecting apurinic/apyrimidine endonuclease 1 (APE1) at the single-cell level. This strategy was applied to assays of lysate samples from HL-7702, HeLa and MCF-7 cells, with a detection limit of lower than 1 HeLa cell.

Keyphrases

single cell
label free
high throughput
cell cycle arrest
rna seq
dna repair
induced apoptosis
loop mediated isothermal amplification
cell death
pi k akt
living cells
nucleic acid
dna damage
cell therapy
stem cells
cell proliferation
mass spectrometry
oxidative stress