Anterograde monosynaptic transneuronal tracers derived from herpes simplex virus 1 strain H129.
Wen-Bo ZengHai-Fei JiangYa-Dong GangYi-Ge SongZhang-Zhou ShenHong YangXiao DongYong-Lu TianRong-Jun NiYaping LiuNa TangXinyan LiXuan JiangDing GaoMichelle AndroulakisXiao-Bin HeHui-Min XiaYing-Zi MingYouming LuJiang-Ning ZhouChen ZhangXue-Shan XiaYousheng ShuShao-Qun ZengFuqiang XuFei ZhaoMin-Hua LuoPublished in: Molecular neurodegeneration (2017)
With the assistance of the helper virus complimentarily expressing TK, H129-ΔTK-tdT replicates in the initially infected neuron, transmits anterogradely through one synapse, and labeled the postsynaptic neurons with tdTomato. The H129-ΔTK-tdT anterograde monosynaptic tracing system offers a useful tool for mapping the direct output in neuronal circuitry. H129-G4 is an anterograde multisynaptic tracer with a labeling signal strong enough to display the details of neuron morphology.